Process for the determination of antibodies which are...

G - Physics – 01 – N

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G01N 33/53 (2006.01) G01N 33/537 (2006.01) G01N 33/543 (2006.01) G01N 33/576 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2001253

The present invention provides a method for the determination in body fluids of antibodies of a definite immunoglobulin class which are directed against an antigen A according to the immunoassay principle by incubation of the sample solution with at least two receptors R1 and R2 which R1 is immobilizable and binds with the antibody to be determined and R2 is labelled and binds either with the antibody to be determined or with the antigen A, separation of the solid phase from the liquid phase and measurement of the label in one of the two phases, wherein the immunological reaction is carried out in homogeneous phase and the sample solution is incubated simultaneously with receptor R1 and an excess of human antibodies or fragments thereof which belong to the same immunoglobulin class as the antibody to be determined and are not bindable with the antigen A and subsequently receptor R2 and possibly further receptors are added thereto. The present invention also provides a reagent kit for carrying out this method.

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