Large scale production of human or animal proteins using...

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/82 (2006.01) C07K 14/47 (2006.01) C07K 16/18 (2006.01) C12N 9/64 (2006.01) C12N 15/57 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2300383

The present invention relates to an expression vector for the large scale production of a human or animal protein, which comprises a DNA construct consisting of operatively linked DNA coding for a plant promoter, a transcription terminator and the human or animal protein to be expressed. Such human or animal proteins may be selected from the group consisting of human protein C (HPC), factor VIII, growth hormone, erythropoietin, interleukin 1 to 7, colony stimulating factors, relaxins, polypeptide hormones, cytokines, growth factors and coagulation factors. The present invention also relates to the plant bioreactor and to the method for the large scale production of human or animal proteins.

La présente invention concerne un vecteur d'expression destiné à la production à grande échelle d'une protéine humaine ou animale, lequel vecteur comprend une construction d'ADN composée d'ADN lié de manière fonctionnelle et codant pour un promoteur de végétal, un codon non-sens de transcription, ainsi que la protéine humaine ou animale à exprimer. On peut choisir de telles protéines humaines ou animales dans le groupe constitué par la protéine humaine C (HPC), le facteur VIII, l'hormone de croissance, l'érythropoïétine, l'interleukine 1 à 7, des facteurs de stimulation de colonies, des relaxines, des hormones polypeptidiques, des cytokines, des facteurs de croissance et des facteurs de coagulation. La présente invention concerne également le bioréacteur pour végétaux ainsi que le procédé de production à grande échelle de ces protéines humaines ou animales.

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