Fluorescent assay for topoisomerase inhibitors

Details Fluorescent assay for topoisomerase inhibitors Fluorescent assay for topoisomerase inhibitors

C - Chemistry, Metallurgy

12

Q

C12Q 1/533 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2324344

The subject invention involves methods for determining the activity of test compounds as topoisomerase inhibitors comprising mixing, in a fluid, a test compound, a DNA, and a topoisomerase; incubating the fluid; then incorporating a cyanine nucleic acid dye such as PicoGreen, measuring the fluorescence, and comparing to the fluorescence from controls.

L'invention concerne des procédés servant à déterminer l'activité de composés d'essai en tant qu'inhibiteurs de topoisomérase et consistant à mélanger, dans un liquide, un composé d'essai, un A D N et une topoisomérase; à incuber le liquide, à incorporer un colorant d'acide nucléique à base de cyanine, tel que PicoGreen?, à mesurer la fluorescence et à comparer cette dernière à la fluorescence émise par des témoins.

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