Methods of capturing species from liquids and assay procedures

G - Physics – 01 – N

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G01N 33/543 (2006.01) C12Q 1/24 (2006.01) C12Q 1/68 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2189958

A species such as a microorganism, e.g. Legionella, Giardia or Cryptosporidium, is captured by first attracting plastics coated magnetic beads or other magnetically attractable particles to a solid support such as stainless steel mesh, which particles have a selective affinity for the species e.g, by virtue of an antibody coating, and contacting a sample containing the species with the particles on the solid support. The beads bearing the capture species may be released by reduction of the magnetic attraction of the support for the beads, e.g. by turning off an electromagnet used to magnetise the support.

On capture une espèce telle qu'un micro-organisme, par exemple Legionella, Giardia ou Cryptosporidium, en attirant d'abord sur un support solide, tel qu'un tamis en acier inoxydable, des perles magnétiques enrobées de matière plastique ou d'autres particules susceptibles d'être attirées magnétiquement, ces particules possédant une affinité sélective pour l'espèce en question, par exemple en vertu d'un revêtement anticorps, puis en mettant en contact un échantillon contenant ladite espèce avec les particules se trouvant sur le support solide. Les perles portant l'espèce capturée peuvent être libérées en réduisant l'attraction magnétique exercée par le support sur celles-ci, par exemple en coupant l'électro-aimant utilisé pour magnétiser le support.

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