Method and apparatus for the genetic detection of...

C - Chemistry – Metallurgy – 12 – N

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150/8.5

C12N 1/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 1072427

ABSTRACT OF THE DISCLOSURE A method and a test kit for the genetic detection of specific microorganisms based on the phenomenon of transformation are disclosed. Variants such as auxotrophs which are distinguishable from their wild, parent strain are transformed by deoxyribonucleic acid (DNA) extracted from micro- organism cells present in a biological test sample. The detection of trans- formed cells indicates the presence of the specific microorganism under deter- mination in the sample. The transforming-DNA is in a sterile, crude form, thereby providing a simple, rapid, and highly specific test method.

209070

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