Primer extension methods utilizing donor and acceptor...

Details Primer extension methods utilizing donor and acceptor... Primer extension methods utilizing donor and acceptor...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2352456

Methods are provided for selective nucleic acid sequence detection in primer extension reactions of high specificity. These methods are useful for detecting small amounts of mutant nucleic acid in a heterogeneous biological sample. These methods are particularly useful for identifying individuals with gene mutations indicative of early colorectal cancer.

L'invention concerne des méthodes de détection sélective d'une séquence d'acide nucléique dans des réactions d'extension d'amorces à haute spécificité. Ces méthodes s'utilisent pour la détection de petites quantités d'acide nucléique mutant dans un prélèvement biologique hétérogène. Ces méthodes sont particulièrement utiles pour l'identification d'individus présentant des mutations géniques indiquant un cancer colorectal précoce.

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