Gene switch

C - Chemistry – Metallurgy – 12 – N

Patent

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47/4, 195/1.2, 1

C12N 15/82 (2006.01) C12N 9/10 (2006.01) C12N 15/67 (2006.01)

Patent

CA 2008695

1. A gene promoter sequence which responds to application of an exogenous chemical inducer such as one of the plant herbicide safeners is utilised as a gene switch to enable external control of expression of a gene placed under control of the promoter. A specific example of the gene switch construct is the maize GSTII (glutathione-S-transferase isoform II) enzyme promoter sequence which is inducer by external application of the safener N,N-diallyl-2,2-dichloroacetamide.

Une séquence de promoteur de gène, qui répond à l'application d'un inducteur chimique exogène, comme par exemple une antidote d'herbicide, est utilisée comme commutation génique pour permettre le contrôle externe de l'expression d'un gène placé sous le contrôle du promoteur. Un exemple spécifique de gène synthétique de commutation est la séquence de promoteur de l'enzyme GSTII du maïs (isoforme II de la glutathione-S-transférase), qui est induite par l'application externe de l'antidote N,N-diallyl-2,2-dichloroacétamide.

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