Microarray synthesis and assembly of gene-length...

Details Microarray synthesis and assembly of gene-length... Microarray synthesis and assembly of gene-length...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2498746

There is disclosed a process for in vitro synthesis and assembly of long, gene- length polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in situ on a microarray platform. Specifically, there is disclosed a process for in situ synthesis of oligonucleotide fragments on a solid phase microarray platform and subsequent, "on device" assembly of larger polynucleotides composed of a plurality of shorter oligonucleotide fragments.

Cette invention se rapporte à un procédé pour la synthèse et l'assemblage <i>in vitro</i> de polynucléotides longs, de la longueur d'un gène, sur la base de l'assemblage de multiples oligonucléotides plus courts synthétisés <i>in situ</i> sur une plate-forme de microréseaux. Plus spécifiquement, cette invention concerne un procédé pour la synthèse <i>in situ</i> de fragments d'oligonucléotides sur une plate-forme de microréseaux en phase solide, puis l'assemblage sur la plate-forme de polynucléotides plus grands composés de plusieurs fragments d'olignucléotides plus courts.

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