Detection method

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) C07H 21/00 (2006.01)

Patent

CA 2060054

A B S T R A C T DETECTION METHOD A method for detecting the presence or absence of more than one variant nucleotide in a target base sequence comprised in a nucleic acid sample using diagnostic primers having a terminal nucleotide complementary to either a suspected variant nucleotide or the corresponding normal nucleotide in the target base sequence and wherein the extension products of more than one diagnostic primer comprise a complementary overlap. Primers and kits for use in the claimed method.

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