Methods and products for peptide-based dna sequence...

G - Physics – 01 – N

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G01N 33/48 (2006.01) C12N 15/09 (2006.01) C12P 21/06 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2355134

A nucleic acid fragment of interest is incorporated into a hybrid artificial gene and expressed in one or more reading frames to produce one or more hybrid polypeptides. The polypeptides are examined with respect to one or more physical parameters, such as mass or amino acid composition. The observed parameter values are used to search a data set of predicted parameter values generated by hypothetical translation of a larger reference nucleic acid sequence so as to determine whether or not the fragment is contained within the reference sequence, and, if it is contained therein, to determine its sequence and/or coding capacity.

L'invention concerne un fragment d'acide nucléique incorporé dans un gène artificiel hybride et exprimé dans un ou plusieurs cadres de lecture de manière à produire un ou plusieurs polypeptides hybrides. Les polypeptides sont examinés sous l'angle d'un ou plusieurs paramètres physiques (par exemple, masse ou composition d'acides aminés). Les valeurs des paramètres examinés sont utilisées pour rechercher un ensemble de paramètres prévus résultant de la traduction hypothétique d'une séquence nucléotidique de référence plus importante, de manière à déterminer si le fragment est contenu dans la séquence de référence et, si tel est le cas, de manière à déterminer la séquence et/ou la capacité de codage de ce fragment.

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