Single recombination system and methods of use

Details Single recombination system and methods of use Single recombination system and methods of use

C - Chemistry, Metallurgy

12

N

C12N 15/863 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2746009

The present invention is directed to a modified poxvirus vector that allows for the generation of recombinant poxviruses by a single recombination event. A modified poxvirus vector comprising at least one reporter gene located between two flanking sequences for homologous recombination is disclosed. Furthermore, a host cell comprising said vector and a method for the generation of recombinant poxviruses using said vector are provided.

La présente invention concerne un vecteur de poxvirus modifié permettant la génération de poxvirus recombinants par le biais d'un événement de recombinaison unique. La présente invention concerne également un vecteur de poxvirus modifié comprenant au moins un gène marqueur encadré par deux séquences pour recombinaison homologue. La présente invention concerne en outre une cellule hôte comprenant ledit vecteur et une méthode de génération de poxvirus recombinants à l'aide dudit vecteur.

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