Methods, systems and compositions for increased...

C - Chemistry – Metallurgy – 12 – N

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C12N 1/21 (2006.01) C12N 15/09 (2006.01) C12P 7/52 (2006.01)

Patent

CA 2731509

The present invention relates to methods, systems and compositions, including genetically modified microorganisms, adapted to exhibit increased tolerance to 3-hydroxypropionic acid (3-HP), particularly through alterations to interrelated metabolic pathways identified herein as the 3-HP toleragenic pathway complex ("3HPTGC"). In various embodiments these organisms are genetically modified so that an increased 3-HP tolerance is achieved. Also, genetic modifications may be made toprovide at least one genetic modification to any of one or more 3-HP biosynthesis pathways in microorganisms comprising one or more genetic modifications of the 3HPTGC.

Cette invention concerne des procédés, des systèmes et des compositions, y compris des micro-organismes génétiquement modifiés, adaptés pour présenter une plus grande tolérance à l'acide 3-hydroxypropionique (3-HP), en particulier par des modifications des voies métaboliques reliées et identifiées ici sous le nom de complexe des voies de 3-HP tolérogènes (3HPTGC). Dans différents modes de réalisation, ces organismes sont génétiquement modifiés pour obtenir une meilleure tolérance à 3-HP. Des modifications génétiques peuvent aussi être réalisées pour obtenir au moins une modification génétique de l'une quelconque au moins des voies de la biosynthèse de 3-HP chez les micro-organismes comprenant une ou plusieurs modifications génétiques du complexe 3HPTGC.

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