Optimized t-dna transfer and vectors therefor

Details Optimized t-dna transfer and vectors therefor Optimized t-dna transfer and vectors therefor

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) A01H 1/00 (2006.01) A61K 48/00 (2006.01) C12N 15/80 (2006.01) C12N 15/81 (2006.01)

Patent

CA 2394840

The present invention relates to T-DNA vectors and methods for obtaining transgenic eukaryotes using said vectors. The transgenic eukaryotes are characterized in that they contain the T-DNA but not the illegitimately transferred vector backbone sequence. This is achieved by modifying the T-DNA borders such that they are more efficiently nicked or such that they allow elimination of illegitimately transferred vector backbone sequences by means of recombination.

La présente invention se rapporte à des vecteurs d'ADN-T et à des méthodes de production d'eucaryotes transgéniques à partir desdits vecteurs. Les eucaryotes transgéniques se caractérisent en ce qu'ils contiennent l'ADN-T mais pas la séquence squelette du vecteur transféré illégitimement. Ceci résulte d'une modification des frontières de l'ADN-T de sorte que celles-ci soit plus efficacement entaillées ou de sorte qu'elles permettent l'élimination des séquences squelette du vecteur transféré illégitimement par recombinaison.

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