C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 1/00 (2006.01) A23L 3/3463 (2006.01) C07H 21/00 (2006.01) C07K 14/00 (2006.01) C07K 14/32 (2006.01) C12N 15/00 (2006.01) C12P 21/00 (2006.01) A61K 38/00 (2006.01)
Patent
CA 2356955
The present invention relates to stable subtilin and methods of producing it. A preferred method comprises one or more of the following steps: applying an acidified composition, comprising a subtilin having a naturally-occuring amino acid sequence, to a hydrophobic interaction column under conditions effective for the subtilin to bind to said column; and eluting said subtilin from the column with an effective elution reagent to form a composition comprising stable subtilin.
La présente invention concerne de la subtiline stable et ses procédés de préparation. Un procédé préféré de réalisation consiste à suivre une ou plusieurs de ces opérations: déposer d'abord une composition acidifiée, contenant de la subtiline à séquence d'acide aminé d'origine naturelle, dans une colonne d'interaction hydrophobe dans des conditions permettant à la subtiline de se fixer à ladite colonne; et procéder ensuite à l'élution de la subtiline de la colonne avec un réactif d'élution efficace de manière à former une composition à base de subtiline stable.
Johnson Ernest Peter
University Of Maryland
LandOfFree
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