Modified .alpha.-amylases having altered calcium binding...

Details Modified .alpha.-amylases having altered calcium binding... Modified .alpha.-amylases having altered calcium binding...

C - Chemistry, Metallurgy

12

N

C12N 15/56 (2006.01) C11D 3/386 (2006.01) C12N 9/26 (2006.01) C12N 9/28 (2006.01) C12S 3/12 (2006.01)

Patent

CA 2253589

Novel .alpha.-amylase enzymes are disclosed in which a new calcium binding site is modified by chemically or genetically altering residues associated with that calcium binding site. The novel .alpha.-amylases have altered performance characteristics, such as low pH starch hydrolysis performance, stability and activity profiles.

Nouvelles .alpha.-amylases dans lesquelles un nouveau site de fixation du calcium est modifié par altération chimique ou génétique de résidus associés à ce site de fixation du calcium. Ces nouvelles .alpha.-amylases présentent des caractéristiques modifiées, par exemple une capacité d'hydrolyse de l'amidon à un pH bas, ainsi qu'en matière de stabilité et d'activité.

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