Mutant of microbacterium, a strain 851r, and a process for...

C - Chemistry – Metallurgy – 12 – N

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195/40, 195/122.

C12N 1/20 (2006.01) A61K 35/74 (2006.01)

Patent

CA 2012278

This invention relates to one of microorganisms which is a mutant of Microbacterium named 851R. The main characteristics of this strain are as follows : a Gram-positive bacterium, motionless, with nitrate reduction ability, able to produce H2S, alive in milk heated to 63°C for 30 minutes or to 72°C for 15 minutes. The thallus is of red or orange red colour. However, the colour of the thallus is turned to pink at about 38°C and to colourless at 40°C. In particular, the DNA of the strain contains R fragment which is detectable with a special R fragment DNA probe. The invention also relates to an industrial process for producing single cell protein nutrient solution by an application of the bacterium. In this process, a Microbacterium strain 851R being cultivated in this invention is the producing strain, a soybean medium or starch medium acts mainly as the producing medium.

Cette invention a trait à l'un des micro-organismes qui est un mutant de Mycobacterium appelé 851R. Les principales caractéristiques de cette souche sont les suivantes : bactérie Gram positive, immobile, capable de réduire les nitrates, capable de produire du H2S, vivant dans du lait chauffé à 63 degrés C pendant 30 minutes ou à 72 degrés C pendant 15 minutes. Le thalle est de couleur rouge ou rouge orangé. Cependant il vire au rose lorsqu'il est chauffé à 38 degrés C et perd sa couleur à 40 degrés C. En particulier, l'ADN de la souche contient le fragment R qui peur être détecté avec une sonde d'ADN spéciale du fragment R. L'invention a également trait à un procédé industriel de production d'une solution nutritive protéique unicellulaire par l'application de la bactérie. Dans ce procédé, une souche de mycobactérie 851R cultivée dans cette invention est la souche productrice, un milieu de soya ou d'amidon sert principalement de milieu de production.

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