C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07H 19/048 (2006.01) C07H 19/06 (2006.01) C07H 19/10 (2006.01) C07H 19/16 (2006.01) C07H 19/20 (2006.01) C07H 21/04 (2006.01) C12Q 1/70 (2006.01)
Patent
CA 2426196
A process for identifying a compound which inhibits viral replication that includes contacting nucleic acids from a virus infected host with an amplification reaction mixture that contains at least two primers and/or probes that provide detectable signals during a polymerase chain reaction, wherein the first primer and/or probe provides a detectable signal on the occurrence of the transcription of viral nucleic acids; and the second primer and/or probe provides a second detectable signal on the occurrence of the transcription of host nucleic acids.
L'invention concerne un procédé pour identifier un composé inhibant la réplication virale. Ce procédé consiste à mettre en contact des acides nucléiques d'un hôte virosé avec un mélange réactionnel d'amplification qui contient au moins deux amorces et/ou sondes émettant des signaux détectables au cours d'une amplification en chaîne par polymérase. Selon cette invention, la première amorce et/ou sonde émet un signal détectable lors de la transcription d'acides nucléiques viraux et la seconde amorce et/ou sonde émet un second signal détectable lors de la transcription d'acides nucléiques hôtes.
Otto Michael J.
Stuyver Lieven
Mbm Intellectual Property Law Llp
Pharmasset Inc.
Pharmasset Ltd.
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