Nucleic acid sequencing with solid phase capturable terminators

Details Nucleic acid sequencing with solid phase capturable terminators Nucleic acid sequencing with solid phase capturable terminators

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2341406

Methods of enzymatic nucleic acid sequencing are provided in which solid-phase capturable chain terminators are employed. In the subject methods, sequencing fragments are generated, where the fragments comprise capturable chain terminators. The fragments are then captured on a solid phase and separated from the remaining components of the sequencing reaction. The fragments are then released from the solid phase, size separated and detected to yield sequencing data from which the sequence of the nucleic acid is determined.

Procédés de séquençage enzymatique d'acide nucléique dans lesquels sont employés des codons d'arrêt de chaîne pouvant être capturés sur une phase solide. Selon lesdits procédés sont produits des fragments de séquençage qui comportent des codons d'arrêt de chaîne pouvant être capturés. Ces fragments sont alors capturés sur une phase solide et séparés des constituants restants de la réaction de séquençage. Lesdits fragments sont ensuite enlevés de la phase solide, séparés selon leur taille et détectés pour produire des données de séquençage à partir desquelles la séquence de l'acide nucléique est déterminée.

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