Thermostable y-family polymerases and chimeras

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 9/12 (2006.01) C12N 5/10 (2006.01) C12N 15/54 (2006.01) C12N 15/62 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2567563

The present disclosure is related to thermostable Y-family polymerases, in particular several novel Y-family polymerases and chimeras made therefrom, as well as methods of identifying other Y-family polymerases, methods of generating other chimeric Y-family polymerases, methods of amplifying ancient or damaged DNA, and methods of incorporating fluorescent or modified nucleotides into a DNA molecule.

La présente invention porte sur des polymérases thermostables de la famille Y, en particulier, sur de nouvelles polymérases et chimères de la famille Y obtenues à partir des précédentes, ainsi que sur des méthodes d'identification d'autres polymérases de la famille Y, sur des méthodes de génération d'autres polymérases chimères de la famille Y, sur des méthodes d'amplification d'ADN ancien ou endommagé, et sur des méthodes d'incorporation de nucléotides fluorescents ou modifiés dans une molécule d'ADN.

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