Canine embryonic stem cells

Details Canine embryonic stem cells Canine embryonic stem cells

C - Chemistry, Metallurgy

12

N

C12N 5/0735 (2010.01) C12N 15/873 (2010.01) A01K 67/027 (2006.01) A61K 35/48 (2006.01) A61K 48/00 (2006.01) C07H 21/04 (2006.01) C12N 15/11 (2006.01) C12Q 1/02 (2006.01)

Patent

CA 2520722

The invention relates to canine embryonic stem cells, methods of cultivation and propagation of the cells, and production of differentiated cells. The embryonic stem cells may be obtained by isolating a canine embryo, culturing the embryo in the presence of a feeder layer and one or more proliferation agent, removing a blastocyst outgrowth, and culturing the outgrowth in the presence of a fresh feeder layer.

L'invention concerne des cellules souches embryonnaires canines, des procédés de culture et de propagation de ces cellules et la production de cellules différenciées. Les cellules couches embryonnaires peuvent être obtenues par isolement d'un embryon canin, par culture de cet embryon en présence d'une couche d'alimentation et d'au moins un agent de prolifération, par enlèvement d'une excroissance d'un blastocyte et par culture de cette excroissance en présence d'une couche d'alimentation fraîche.

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