G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 15/04 (2006.01) C12N 5/076 (2010.01) B01D 21/26 (2006.01) B03D 3/00 (2006.01) C12Q 1/24 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2428041
The present invention relates to a method and kit for discontinuous density gradient centrifugation to separate a minimum of two substances in a sample. In the method and kit use is made of colloidal density media and low density solutions, such as buffer solutions. The sample is mixed with colloidal media and placed at the bottom of a centrifuge tube and desired substances flotate to the lower density media following centrifugation.
La présente invention concerne une méthode et une trousse permettant de réaliser une centrifugation en gradient discontinu de densité afin de séparer au moins deux substances dans un échantillon. Pour ce faire, on utilise un milieu colloïdal à densité élevée et une solution à faible densité, telle qu'une solution tampon. Ledit échantillon est mélangé avec le milieu colloïdal et placé au niveau de la partie inférieure d'un tube à centrifugation. A la suite d'une centrifugation, les substances souhaitées flottent à la surface de la solution à faible densité.
Norling Borje
Pertoft Hakan
Amersham Biosciences Ab
Fetherstonhaugh & Co.
Ge Healthcare Bio-Sciences Ab
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