Magnetic separation apparatus and methods

G - Physics – 01 – N

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Details

G01N 33/48 (2006.01) B01D 35/06 (2006.01) B03C 1/28 (2006.01) C12M 1/34 (2006.01) C12M 1/42 (2006.01) C12Q 1/00 (2006.01) G01N 33/533 (2006.01) G01N 33/543 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2352064

Apparatuses and methods for separating, immobilizing, and quantifying biological substances from within a fluid medium. Biological substances are observed by employing a vessel (6) having a chamber therein, the vessel comprising a transparent collection wall (5). A high internal gradient magnetic capture structure may be on the transparent collection wall (5), magnets (3) create an externally-applied force for transporting magnetically responsive material toward the transparent collation wall (5). The magnetic capture structure comprises a plurality of ferromagnetic members and has a uniform or non-nonuniform spacing between adjacent members. There may be electrical conductor means supported on the transparent collection wall (5) for enabling electrical manipulation of the biological substances. The chamber has one compartment or a plurality of compartments with differing heights. The chamber may include a porous wall. The invention is also useful in conducting quantitative analysis and sample preparation in conjunction with automated cell enumeration techniques.

L'invention concerne des appareils et des procédés permettant de séparer, d'immobiliser et de quantifier des substances biologiques dans un milieu fluide. Les substances biologiques sont observées au moyen d'un récipient (6) comportant une chambre interne et une paroi de collecte transparente (5). Ladite paroi (5) peut comprendre une structure de capture magnétique à haut gradient interne, les aimants (3) créant une force appliquée depuis l'extérieur qui transporte la matière magnétisable vers la paroi de collecte transparente (5). La structure de capture magnétique comprend une pluralité d'éléments ferromagnétiques, les éléments adjacents étant espacés les uns des autres de façon uniforme ou non uniforme. La paroi de collecte transparente (5) peut comporter des éléments conducteurs d'électricité permettant la manipulation électrique des substances biologiques. La chambre est composée d'un ou de plusieurs compartiments de hauteur variable. Ladite chambre peut comprendre une paroi poreuse. L'invention est également utile pour effectuer des analyses quantitatives et pour préparer des échantillons, en combinaison avec les techniques automatisées de comptage de cellules.

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