G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/573 (2006.01) A61K 38/04 (2006.01) C07K 1/00 (2006.01) C07K 5/00 (2006.01) C07K 7/00 (2006.01) C07K 14/00 (2006.01) C07K 14/435 (2006.01) C07K 16/00 (2006.01) C07K 17/00 (2006.01) C08H 1/00 (2006.01) C12Q 1/37 (2006.01) G01N 33/53 (2006.01) G01N 33/569 (2006.01) G01N 33/58 (2006.01) G01N 33/60 (2006.01)
Patent
CA 2462686
The present invention provides clostridial toxin substrates useful in assaying for the protease activity of any clostridial toxin, including botulinum toxins of all serotypes as well as tetanus toxins. A clostridial toxin substrate of the invention contains a donor fluorophore; an acceptor having an absorbance spectrum overlapping the emission spectrum of the donor fluorophore; and a clostridial toxin recognition sequence that includes a cleavage site, where the cleavage site intervenes between the donor fluorophore and the acceptor and where, under the appropriate conditions, resonance energy transfer is exhibited between the donor fluorophore and the acceptor.
La présente invention porte sur des substrats de toxines clostridiennes utilisés pour déterminer l'activité de la protéase de n'importe quelle toxine clostridienne, y compris les toxines botuliniques de tous les sérotypes ainsi que les toxines tétaniques. Le substrat de la toxine clostridienne de cette invention renferme un fluorophore donneur; un accepteur dont le spectre d'absorption chevauche le spectre d'émission du fluorophore donneur et une séquence de reconnaissance des toxines clostridiennes qui comprend un site de clivage intervenant entre le fluorophore donneur et l'accepteur et où, dans les conditions appropriées, le transfert de l'énergie de résonance est mis en évidence entre le fluorophore donneur et l'accepteur.
Aoki Kei Roger
Fernandez-Salas Ester
Steward Lance E.
Allergan Inc.
Gowling Lafleur Henderson Llp
LandOfFree
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