Novel phenol oxidizing enzymes

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/53 (2006.01) C12N 1/15 (2006.01) C12N 9/02 (2006.01)

Patent

CA 2384954

Disclosed herein are novel phenol oxidizing enzymes naturally-produced by strains of the species Stachybotrys which possess a pH optima in the alkaline range and which are useful in modifying the color associated with dyes and colored compounds, as well as in anti-dye transfer applications. Also disclosed herein are biologically-pure cultures of strains of the genus Stachybotrys, designated herein Stachybotrys parvispora MUCL 38996 and Stachybotrys chartarum MUCL 38898, which are capable of naturally-producing the novel phenol oxidizing enzymes. Disclosed herein is the amino acid and nucleic acid sequence for Stachybotrys phenol oxidizing enzymes B as well as expression vectors and host cells comprising the nucleic acid. Disclosed herein are methods for producing the phenol oxidizing enzyme as well as methods for constructing expression hosts.

La présente invention concerne de nouvelles enzymes oxydant le phénol produites naturellement par des souches de l'espèce Stachybotrys, qui possèdent un pH optimal dans la plage alcaline et qui sont utilisées pour modifier la couleur associée à des colorants et à des composés colorés, ainsi que dans les applications de protection contre le transfert pigmentaire. L'invention se rapporte également à des cultures biologiquement pures de souches du genre Stachybotrys, désignées selon l'invention par Stachybotrys parvispora MUCL 38996 et Stachybotrys chartarum MUCL 38898, qui sont capables de produire naturellement les nouvelles enzymes oxydant le phénol. L'invention concerne également l'acide aminé et la séquence nucléotidique de l'enzyme B oxydant le phénol de Stachybotrys, ainsi que les vecteurs d'expression et les cellules hôtes renfermant l'acide nucléique précité. L'invention se rapporte enfin à des procédés permettant de produire les enzymes oxydant le phénol ainsi qu'à des procédés de construction des hôtes d'expression.

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