Determination of glycated proteins

Details Determination of glycated proteins Determination of glycated proteins

C - Chemistry, Metallurgy

12

Q

C12Q 1/26 (2006.01) C12Q 1/28 (2006.01) C12Q 1/37 (2006.01)

Patent

CA 2218982

Inter alia, a method for the determination of a glycated protein in a sample characterised in that it comprises: mixing the sample and a first reagent containing a proteinase and a peroxidase so as to produce a substrate capable of oxidation by a ketoamine oxidase; adding a second reagent containing a ketoamine oxidase; and measuring hydrogen peroxide produced or oxygen consumed so as to detect and/or quantify the glycated protein is disclosed.

L'invention décrit, entre autres, un procédé visant à déterminer la présence d'une protéine glycosylée dans un échantillon, caractérisé par le fait qu'il consiste à mélanger l'échantillon et un premier réactif contenant une protéinase et une peroxydase afin d'obtenir un substrat pouvant être oxydé par une oxydase de la cétamine, à ajouter un second réactif contenant une oxydase de la cétamine, et à mesurer le peroxyde d'hydrogène produit ou l'oxygène consommé afin de détecter et/ou de quantifier la protéine glycosylée.

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