Methods for preparing compositions comprising heat shock...

A - Human Necessities – 61 – K

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Details

A61K 39/39 (2006.01) A61K 39/00 (2006.01) A61K 39/12 (2006.01) A61K 47/42 (2006.01) A61K 47/48 (2006.01) A61P 31/12 (2006.01) A61P 35/00 (2006.01)

Patent

CA 2457008

The present invention relates to methods and compositions for the prevention and treatment of infectious diseases, and cancers. The methods of the invention comprises complexing a population of antigenic proteins or antigenic peptides derived from antigenic cells or viral particles to one or more different heat shock proteins in vitro. The population or the protein preparation used to produce the antigenic peptides comprises at least 50% of the different proteins or at least 50 different proteins of the antigenic cells or viral particles. Methods for making antigenic peptides comprise digesting a protein preparation of antigenic cells, a cellular fraction thereof, or of viral particles with one or more proteases, or exposing the protein preparation to ATP, guanidium hydrochloride, and/or acidic conditions.

L'invention concerne des procédés et des compositions de prévention et de traitement des maladies infectieuses et du cancer. Les procédés de l'invention consistent à complexer, in vitro, une population de protéines ou de peptides antigéniques issus de cellules antigéniques ou de particules virales en une ou plusieurs protéines du stress différentes. La préparation des protéines utilisée pour obtenir des peptides antigéniques contient au moins 50 % desdites protéines différentes ou au moins 50 protéines différentes provenant des cellules antigéniques ou des particules virales. Les procédés de préparation des peptides antigéniques consistent à soumettre à la digestion par une ou plusieurs protéases une préparation protéique de cellules antigènes, une fraction cellulaire de celles-ci, ou une préparation protéique de particules virales, ou encore, à exposer cette préparation protéique à l'ATP, au chlorhydrate de guanidium, et/ou dans des conditions acides.

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