Sulfonylurea-responsive repressor proteins

Details Sulfonylurea-responsive repressor proteins Sulfonylurea-responsive repressor proteins

C - Chemistry, Metallurgy

07

K

C07K 14/195 (2006.01)

Patent

CA 2740147

Compositions and methods relating to the use of sulfonylurea-responsive repressors are provided. Compositions include polypeptides that specifically bind to an operator, wherein the specific binding is regulated by a sulfonylurea compound. Compositions also include polynucleotides encoding the polypeptides as well as constructs, vectors, prokaryotic and eukaryotic cells, and eukaryotic organisms including plants and seeds comprising the polynucleotide, and/or produced by the methods. Also provided are methods to provide a sulfonylurea-responsive repressor to a cell or organism, and to regulate expression of a polynucleotide of interest in a cell or organism, including a plant or plant cell.

La présente invention concerne des compositions et des procédés liés à l'utilisation de répresseurs sensibles à la sulfonylurée. Les compositions comprennent des polypeptides qui se lient spécifiquement à un opérateur, la liaison spécifique étant régulée par un composé de sulfonylurée. Les compositions comprennent également des polynucléotides codant lesdits polypeptides, ainsi que des constructions, des vecteurs, des cellules procaryotes et eucaryotes, et des organismes eucaryotes, notamment des plantes et des graines, comprenant lesdits polynucléotides, et/ou produits par lesdits procédés. L'invention concerne également des procédés permettant d'obtenir un répresseur d'une cellule ou d'un organisme sensible à la sulfonylurée, et de réguler l'expression d'un polynucléotide d'intérêt dans une cellule ou un organisme, notamment une plante ou une cellule végétale.

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