C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/53 (2006.01) A01H 5/00 (2006.01) C07K 14/195 (2006.01) C12N 5/10 (2006.01) C12N 9/02 (2006.01) C12N 15/31 (2006.01) C12N 15/82 (2006.01) C12P 7/64 (2006.01)
Patent
CA 2550028
The present invention provides transgenic plants with reduced levels of saturated fatty acids in the seed oil and methods of making these plants. The transgenic plants developed through this method contain reduced levels of saturated fatty acids in seed oil due to expression of a prokaryotic delta-9 desaturase enzyme (i.e. an enzyme that introduces cis double bonds at the delta-9 position of saturated fatty acids) operably linked with an endoplasmic reticulum retention and retrieval signal sequence. One example of the invention is a plant expressing a heterologous delta-9 desaturase enzyme from cyanobacterium Anacystis nidulans, which converts lipid-bound 16:0 and 18:0 fatty acids into corresponding 16:1 and 18:1, in operative linkage with a KKSS (SEQ ID NO:5) endoplasmic reticulum retention and retrieval signal sequence.
La présente invention concerne des plantes transgéniques présentant une teneur réduite en acides gras saturés dans l'huile de graine, ainsi que leurs méthodes de production. Ces plantes transgéniques ont des niveaux réduits d'acides gras saturés dans l'huile de graine, en raison de l'expression d'une enzyme procaryote, la delta-9 désaturase (c'est-à-dire une enzyme qui introduit des doubles liaisons cis en position delta-9 des acides gras saturés), liée fonctionnellement à une séquence-signal de récupération et de rétention du réticulum endoplasmique. Un exemple de l'invention concerne une plante exprimant une enzyme, la désaturase delta-9 hétérologue, à partir de la cyanobactérie Anacystis nidulans, qui convertit les acides gras 16:0 et 18:0 associés aux lipides en 16:1 et 18:1 correspondants, en liaison fonctionnelle avec une séquence-signal de récupération et de rétention du réticulum endoplasmique (SEQ ID NO:5) KKSS.
Shah Salehuzzaman
Weselake Randall
Alberta Innovates - Technology Futures
Alberta Research Council Inc.
Gowling Lafleur Henderson Llp
LandOfFree
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