Nucleic acid sequence detection

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01)

Patent

CA 2505636

The present application discloses a system and a method for detecting a target nucleic acid sequence using a support 104 comprising an electrode 22 with a nucleic acid probe 24 attached thereto. A nucleic acid target 26 and a non- covalent photoelectrochemical label 30 are contacted with the probe 24. The photoelectrochemical intercalator 30 preferentially binds double-stranded nucleic acids. Irradiating the mixture generates a photocurrent at the electrode 22. Also disclosed is a support 104 comprising an array of nucleic acid probe elements.

La présente invention concerne un système et une méthode de détection d'une séquence d'acides nucléiques cibles au moyen d'un support (104) qui comporte une électrode (22) à laquelle est fixée une sonde d'acides nucléiques (24). Une cible d'acides nucléiques (26) et un marqueur photoélectrochimiuqe non covalent (30) sont mis en contact avec la sonde (24). L'intercalant photoélectrique (30) relie de préférence des acides nucléiques à double brin. L'irradiation du mélange produit un courant photoélectrique au niveau de l'électrode (22). Est également décrit un support (104) qui comprend une batterie d'éléments de sondes d'acides nucléiques.

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