Methods for detecting genetic haplotypes by interaction with...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2463420

The present invention provides methods for determining genetic haplotype comprising identifying target nucleic acids or gene fragments, said nucleic acids or gene fragments comprising a haplotype of interest, and detecting at least one parameter displayed by one of a primer-dependent transcript, a probe, or a primer-dependent transcript/probe complex. Further provided are methods for determining genetic haplotype comprising labeling nucleic acids or gene fragments with a least one probe, and detecting the velocity of the nucleic acid molecule or gene fragment by sequentially measuring at least one parameter displayed by the probes.

L'invention concerne des procédés destinés à déterminer un haplotype génétique. Ces procédés consistent à identifier des acides nucléiques cibles ou des fragments de gène, ces acides nucléiques ou ces fragments de gène comprenant un haplotype d'intérêt, et à détecter au moins un paramètre affiché par un parmi un transcrit dépendant d'amorces, une sonde, ou un complexe transcrit/sonde dépendant d'amorces. En outre, l'invention concerne des procédés destinés à déterminer un haplotype génétique. Ces procédés consistent à marquer des acides nucléiques ou des fragments de gène au moyen d'au moins une sonde, et à détecter la vitesse de la molécule d'acide nucléique ou du fragment de gène par mesure séquentielle d'au moins un paramètre affiché par les sondes.

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