Isolated proteins having retinol dehydrogenase activity, and...

Details Isolated proteins having retinol dehydrogenase activity, and... Isolated proteins having retinol dehydrogenase activity, and...

C - Chemistry, Metallurgy

07

K

C07K 14/435 (2006.01) C12N 9/00 (2006.01) C12N 9/02 (2006.01) C12N 9/04 (2006.01)

Patent

CA 2304508

Isolated membrane associated proteins which have a molecular weight of from about 30 kD to about 36 kilodaltons as determined by SDS-PAGE, are disclosed. The proteins have <u>5</u>cis retinol dehydrogenase activity, such as 9, 11 or 13 cis retinol dehydrogenase activity, or cis activity at more than one position. Other trans-retinol dehydrogenases are also disclosed. Nucleic acid molecules which code for the proteins are also disclosed.

L'invention concerne des protéines isolées associées à la membrane ayant un poids moléculaire compris entre 30 kD environ et 36 kD selon les analyses SDS-PAGE. Lesdites protéines ont une activité de rétinol déshydrogénase en 5-cis, telle qu'une activité de rétinol déshydrogénase en 9-, 11-, ou 13-cis, ou une activité cis au niveau de plus d'une position. L'invention concerne également d'autres trans-rétinol déshydrogénases, ainsi que des molécules d'acide nucléique codantes pour ces protéines.

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