Recombinant dna method and vectors for use therein

Details Recombinant dna method and vectors for use therein Recombinant dna method and vectors for use therein

C - Chemistry, Metallurgy

12

N

C12N 15/85 (2006.01) C12N 5/10 (2006.01) C12N 15/52 (2006.01)

Patent

CA 2071543

There is described a method, for obtaining a eukaryotic cell containing in its DNA multiple copies of a glutamine synthetase (GS) gene, comprising: transforming a eukaryotic glutamine auxotroph with a GS gene; selecting transformant cells containing the GS gene; and culturing the selected transforant cells in a medium which lacks glutamine or in which the amount of glutamine is progressively depleted, the GS gene being of a character such that, or the conditions employed during the culturing step being such that, the GS gene is so weakly transcribed that cells in which the GS gene has been amplified are selected. Also described are DNA vectors for use in the aforementioned method.

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