Test for predicting neutralization of asparaginase activity

Details Test for predicting neutralization of asparaginase activity Test for predicting neutralization of asparaginase activity

C - Chemistry, Metallurgy

12

Q

C12Q 1/37 (2006.01)

Patent

CA 2743020

Method of in vitro measurement of the presence of factors that are able to neutralize asparaginase activity in a sample of blood, plasma, serum or derived medium that may contain asparaginase neutralizing factors, obtained from a patient, comprising mixing of said sample with asparaginase, incubation of said mixture, then measurement of the residual asparaginase activity in the mixture and determination or quantification of the presence of said neutralizing factors. Method for predicting the efficacy of a treatment with asparaginase.

L'invention porte sur une méthode de mesure in vitro de la présence de facteurs qui sont aptes à neutraliser l'activité de l'asparaginase dans un échantillon de sang, de plasma, de sérum ou de milieu dérivé qui peut contenir des facteurs neutralisant l'asparaginase, obtenu d'un patient, comprenant le mélange dudit échantillon avec de l'asparaginase, l'incubation dudit mélange, puis la mesure de l'activité résiduelle de l'asparaginase dans le mélange et la détermination ou la quantification de la présence desdits facteurs neutralisants. L'invention porte sur une méthode pour la prédiction de l'efficacité d'un traitement faisant appel à de l'asparaginase.

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