Solid phase cell lysis and capture platform

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 1/06 (2006.01) C12M 1/40 (2006.01) C12M 3/00 (2006.01)

Patent

CA 2524393

The present invention provides containers, processes, and kits relating to the extraction or the extraction and isolation of a cellular component from a host cell. More specifically, the containers of the invention comprise a mouth; an interior surface comprising a sidewall formation and a bottom; a volume; a lytic reagent; and in some instances, a supported capture ligand. Methods and kits for the extraction or the extraction and isolation of a cellular component from a host cell using the containers described herein are also provided.

L'invention concerne des contenants, des procédés, et des kits relatifs à l'extraction ou l'extraction et l'isolement d'un composé cellulaire issu d'une cellule hôte. Plus précisément, les contenants de l'invention comprennent un bouche ; une surface intérieure équipée d'une paroi latérale et d'un fond ; un volume ; un réactif lytique ; et dans certains cas, un ligand de saisie supporté. L'invention se rapporte aussi à des procédés et des kits d'extraction ou d'extraction et d'isolement d'un composé cellulaire à partir d'une cellule hôte au moyen des contenants susmentionnés.

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