C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/12 (2006.01) C07K 14/435 (2006.01) C07K 14/545 (2006.01) C12N 9/38 (2006.01) C12N 9/86 (2006.01) C12N 15/10 (2006.01) C12N 15/52 (2006.01) C12N 15/64 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2239099
A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
L'invention porte sur une méthode de réassemblage d'ADN après sa fragmentation aléatoire et son application à la mutagenèse de séquences d'acide nucléique par recombinaison in vitro ou in vivo. Elle porte en particulier sur une méthode d'obtention de fragments d'acide nucléique ou de polynucléotides codant pour des protéines mutantes. Elle porte en outre sur une méthode de répétition de cycles de mutagenèse, de brassage et de sélection permettant une évolution dirigée in vitro ou in vivo des protéines.
Crameri Andreas
Stemmer Willem P. C.
Affymax Technologies N.v.
Maxygen Inc.
Smart & Biggar
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