Megsin promoter

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/63 (2006.01) C07K 14/00 (2006.01) C07K 14/47 (2006.01) C12N 5/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2358928

A genomic DNA region containing a part of about 1.5 kb upstream of MEGSIN is isolated and the base sequence thereof is determined. Two regulatory sequences positively regulating transcription in this genomic DNA region are identified. A promoter region located in the 3'-side, between the above two regulatory sequences, is subjected to base substitution by the site-directed mutagenesis method and thus the base sequence playing an important role in the promoter activity is successfully specified.

La présente invention concerne un région génomique d'ADN comprenant une partie d'environ 1.5 kb en amont du gène MEGSINE isolée et dont la séquence de base est déterminée. Deux régions régulatrices de régulation positive de la transcription génomique dans ladite région génomique de l'ADN sont identifiées. Une région promotrice située dans le côté 3', entre lesdites régions régulatrices, est soumise à la substitution de base par le procédé de mutagenèse dirigée causant ainsi la séquence de base jouant un rôle important dans l'activité de promotion d'être déterminée.

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