C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/06 (2006.01) A61K 38/18 (2006.01) A61L 27/00 (2006.01) A61L 27/22 (2006.01) A61L 27/34 (2006.01) A61L 27/38 (2006.01) A61L 29/00 (2006.01) A61L 29/08 (2006.01) A61L 31/00 (2006.01) A61L 31/10 (2006.01) C07K 14/47 (2006.01) C07K 14/78 (2006.01) C07K 16/18 (2006.01) C12N 5/08 (2006.01)
Patent
CA 2174735
A method for growing epithelial cells in vitro using soluble proteins secreted by 804G rat bladder carcinoma cells. These proteins are able to stimulate cell attachment and hemidesmosome formation in cells grown in contact with the proteins. The purification of these proteins from 804G culture supernatant is greatly facilitated by culturing the cells under low serum conditions. Trans-epithelial appliances coated with the soluble proteins and the use of the soluble proteins in the maintenance of human tissue ex vivo are also disclosed.
Procédé de culture in vitro de cellules épithéliales à l'aide de protéines solubles sécrétées par les cellules cancéreuses 804G de la vessie du rat. Ces protéines peuvent stimuler la fixation cellulaire et la formation d'hémidesmosomes dans les cellules cultivées au contact des protéines. On peut considérablement faciliter la purification de ces protéines à partir du surnageant de la culture des cellules 804G en cultivant les cellules dans des conditions caractérisées par un faible taux de sérum. On a également prévu des appareils trans-épithéliaux enrobés desdites protéines solubles, ainsi que l'utilisation de ces protéines solubles dans la conservation ex vivo des tissus humains.
Hormia Marketta
Quaranta Vito
Cythera Inc.
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
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