C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/63 (2006.01) C07K 14/16 (2006.01) C12N 9/12 (2006.01) C12N 9/50 (2006.01) C12N 15/00 (2006.01) C12N 15/48 (2006.01) C12N 15/57 (2006.01) C12N 15/70 (2006.01) A61K 38/00 (2006.01)
Patent
CA 2059541
The present invention provides a method of preparing plasmids having the ability of expressing retroviral genes and/or effecting the post- translational processing of the encoded products, and the resultant plasmid and the expression products thereof. A method of the present invention is to prepare a plasmid by insertion-linking a cDNA fragment containing at least the protease gene from among retroviral genes with a highly expressing gene or an expression vector for the direct expression of its gene products, thereby causing expression of the retroviral genes, and at the same time, to process said expression product itself with the protease in that expression product, thereby mass-producing the retroviral genes. These retroviral genes comprise three kinds of core protein encoded by gag gene, and three kinds of enzymes encoded by pol gene, in the form of individual single mature or active proteins. Alternatively, the method of the present invention is to prepare a plasmid in which a cDNA fragment containing HIV nef gene region is inserted into a highly expressing gene and an expression vector for the direct expression of its gene products, thereby mass-producing Nef protein which is the expression product of nef gene.
Nakata Atsuo
Saito Atsusi
Shinagawa Hideo
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
The Research Foundation For Microbial Diseases Of Osaka Universi
LandOfFree
Method of preparing a plasmid having both the ability to... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of preparing a plasmid having both the ability to..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of preparing a plasmid having both the ability to... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1694761