Methods and kits for evaluating and measuring the...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/02 (2006.01) G01N 33/15 (2006.01) C12N 5/09 (2010.01)

Patent

CA 2690751

Three-dimensional physiological matrices, methods, apparatus and kits for the expedited design, testing and evaluation of oncological remedies are provided. Key aspects of the inventions include matrices, and especially gel matrices, comprising one or more physiological fibers, which are adapted and arranged to provide conditions which permit behaviors, such as the movement of cells away from the margins of samples of target tissue through the matrix, to be evaluated in a manner that produces data useful for evaluating the oncological status and characteristics of the cells. In a further key aspect, the invention permits the in vitro testing and analyses of one or more conventional, experimental or theoretical therapies with respect to specific target tissues or cells. Among such therapies are therapeutic compounds and combinations thereof, radiation therapies, combinations of therapeutic compounds and radiation and numerous other possible therapies.

La présente invention concerne des matrices physiologiques en trois dimensions, des procédés, un appareil et des kits pour la conception, le test et l'évaluation accélérés de remèdes oncologiques. Les principaux aspects de l'invention concernent des matrices, et plus particulièrement des matrices gels, comprenant une ou plusieurs fibres physiologiques, qui sont adaptées et organisées pour réunir les conditions permettant des comportements, tels que le mouvement de cellules depuis les marges des prélèvements de tissu cible au travers de la matrice, à évaluer d'une manière générant des données utiles pour l'évaluation du statut oncologique et des caractéristiques des cellules. Dans un autre aspect important, l'invention permet le test et les analyses in vitro d'un ou plusieurs traitements traditionnels, expérimentaux ou théoriques sur des tissus ou des cellules cibles spécifiques. Parmi ces traitements, on peut citer des composés thérapeutiques et des combinaisons de ceux-ci, des radiothérapies, des combinaisons de composés thérapeutiques et de radiothérapie et de nombreux autres traitements possibles.

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