Enzyme-linked immuno-magnetic electrochemical biosensor

G - Physics – 01 – N

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G01N 27/327 (2006.01) C12Q 1/00 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2364288

A electrochemical biosensor system based on enzyme-linked immuno-magnetic sandwich assay wherein an interdigitated array of electrodes is equipped with a magnet to attract magnetic beads. Magnetic particles bear a first recognition molecule capable of binding to the analyte. Enzymes are chemically modified to complex with the analyte. When the sandwich assay is performed, a substrate is added. The substrate is chosen such that it is cleavable by the enzyme in a reporting molecule capable of redox recycling. A substrate when cleaved preferably leads to a p-aminophenol.

Cette invention concerne un biocapteur électrochimique reposant sur une analyse immuno-magnétique à liaison enzymatique <= en sandwich >= dans laquelle un réseau d'électrodes interdigitées est muni d'un aimant qui attire des billes magnétiques. Ces particules magnétiques portent une première molécule de reconnaissance capable de se lier à l'analyte. On modifie chimiquement des enzymes pour qu'ils forment un complexe avec l'analyte. Lors de l'exécution de l'analyse en sandwich, on ajoute un substrat. Le substrat choisi doit pouvoir être clivé en une molécule rapporteur capable de recyclage redox. Au clivage, ce substrat doit de préférence donner un p-aminophénol.

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