Novel fab fragment libraries and methods for their use

Details Novel fab fragment libraries and methods for their use Novel fab fragment libraries and methods for their use

C - Chemistry, Metallurgy

12

N

C12N 15/63 (2006.01) C07H 21/00 (2006.01) C07K 16/00 (2006.01) C07K 16/18 (2006.01) C07K 16/26 (2006.01) C07K 16/28 (2006.01) C12N 15/09 (2006.01) C12N 15/13 (2006.01) C12N 15/70 (2006.01) C12P 19/34 (2006.01) C12P 21/08 (2006.01) C40B 40/08 (2006.01) C40B 40/10 (2006.01) G01N 33/563 (2006.01)

Patent

CA 2372582

The present invention provides Fab libraries and methods for using the Fab libraries to obtain antibodies against a target. The Fab library of the invention contains at least 109 different Fabs, and in some embodiments, at least 1010 different Fabs. The Fab libraries of the invention are used to isolate polyclonal or monoclonal Fabs that bind with high specificity to targets.

La présente invention concerne des bibliothèques de fragments Fab et des procédés d'utilisation de ces bibliothèques pour obtenir des anticorps contre une cible. La bibliothèque selon l'invention contient au moins 10?9¿ fragments Fab différents, et dans certains modes de réalisation, au moins 10?10¿ fragments Fab différents. Les bibliothèques selon l'invention sont utilisées pour isoler des fragments Fab polyclonaux ou monoclonaux qui se lient avec une spécificité élevée aux cibles.

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