Method of labelling crnas for probing oligo-based microarrays

Details Method of labelling crnas for probing oligo-based microarrays Method of labelling crnas for probing oligo-based microarrays

C - Chemistry, Metallurgy

12

P

C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2450215

A novel method of labelling cRNAs for probing oligo-based microarrays is disclosed. The polynucleotide template of interest is reverse transcribed into double-stranded cDNA using random primers that include an RNA polymerase promoter. This cDNA is then used as a template for synthesis of labeled RNA via in vitro transcription in the presence of labeled precursors.

Nouveau procédé de marquage d'ARNc utile pour sonder des microréseaux d'oligodésoxyribonucléotides. On soumet le modèle de polynucléotide voulu à une transcription inverse pour obtenir de l'ADNc bicaténaire en utilisant des amorces aléatoires incluant un promoteur d'ARN polymérase. Cet ADNc sert ensuite de modèle pour la synthèse d'ARN marqué par transcription in vitro en présence de précurseurs marqués.

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