Conditioned culture medium for rapid growth and detection of...

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/04 (2006.01) C12M 1/34 (2006.01) C12N 1/20 (2006.01) C12Q 1/06 (2006.01) C12Q 1/10 (2006.01)

Patent

CA 2189756

This invention generally relates to products and processes used to determine the presence of microbes in a sample and particularly relates to a conditioned culture medium which may be used in products and processes to allow early detection and enumeration of such microbes. The conditioned culture medium preferably is a filtered broth made by incubating viable microbes with gelatin, casein or animal peptones as well as lactose, sodium chloride, bile salts, guar gum and an indicator.

La présente invention concerne d'une manière générale des produits et des procédés servant à mettre en évidence la présence de microbes dans un échantillon. L'invention concerne plus particulièrement un milieu de culture conditionné utilisable pour les produits et procédés permettant de procéder précocement à la mise en évidence et à la numération de tels microbes. Dans une réalisation préférée, le milieu de culture conditionné est un bouillon filtré obtenu par incubation de microbes viables dans de la gélatine, de la caséine ou des peptones d'origine animale ainsi que du lactose, du chlorure de sodium, des sels biliaires, de la gomme guar et un indicateur.

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