Culture system for mouse tracheal epithelial cells

C - Chemistry – Metallurgy – 12 – N

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C12N 5/02 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2445961

Cultured mouse tracheal epithelial are grown in an air/liquid interface culture that allows them to develop differentiated characteristics (i.e., to develop into mucus cells or ciliated cells). This invention may be used for the growth of cells isolated from knockout and transgenic mice. The primary culture cells of the culture may be ciliated or non-ciliated differentiated cells.

L'invention porte sur des cellules d'épithélium trachéal de souris proliférant dans une culture à interface air/liquide leur permettant de développer des caractéristiques différenciées (c.-à-d. en cellules de muqueuse ou en cellules ciliées). L'invention peut s'appliquer à la mise en culture de cellules isolées à partir de souris knockout et transgéniques. Les cellules initiales de la culture peuvent être des cellules différenciées ciliées ou non ciliées.

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