Nucleic acid sequencing method

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C07H 19/04 (2006.01) C07H 21/00 (2006.01)

Patent

CA 2355365

A method of determining the sequence of a nucleic acid involves the use of an excimer or exciplex as a label. The method may comprise effecting a single copying reaction of the nucleic acid to be sequenced using chain extending nucleotides and an appropriate number of chain terminating nucleotides each labelled with the partners of a detectably different excimer or exciplex, the method further comprising separating the labelled fragments produced and detecting the labels to determine the sequence of the nucleic acid.

L'invention concerne un procédé permettant de déterminer la séquence d'un acide nucléique, qui implique l'utilisation, comme marqueur, d'un excimère ou d'un exciplexe. Le procédé peut consister à effectuer une réaction de copiage simple de l'acide nucléique à séquencer au moyen de nucléotides d'allongement de chaîne et d'un nombre approprié de nucléotides de terminaison de chaîne, chacun marqué par des partenaires d'un excimère ou d'un exciplexe différent de manière détectable. Le procédé consiste également à séparer les fragments marqués produits et à détecter les marqueurs afin de déterminer la séquence de l'acide nucléique.

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