Method for isolating a portion of a layer of a biological...

G - Physics – 01 – N

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Details

G01N 1/06 (2006.01) G01N 1/28 (2006.01)

Patent

CA 2398144

The invention relates to a film (14) comprising a tissue specimen (10) which is placed face down with said tissue specimen (10) onto a microscope object holder (18). Afterwards, the microscope object holder (18) is positioned in the object plane of an inverse microscope. An adhesive strip (20), with an adhesive (22), is placed face down above the film (14) and, as a result, above the tissue specimen (10). In the next step, the tissue (36) to be isolated is cut out by a focussed laser beam. This also results in severing the film (14). The adhesive strip (20) is subsequently removed from the microscope. The cut- out tissue pieces (36) adhere to the adhesive strip (20). The rest of the film (14) and the tissue specimen (10) remain adhered to the microscope object holder (18).

L'invention concerne une pellicule (14) comportant une coupe tissulaire (10), qui est placée conjointement avec ladite coupe tissulaire (10), vers le bas, sur une lame porte-objects de microscope (18). Ladite lame porte-objects de microscope (18) est ensuite positionnée dans le plan d'objet d'un microscope inverse. Une bande adhésive (20) est disposée avec un adhésif (22), vers le bas, au-dessus de la pellicule (14) et par conséquent au-dessus de la coupe tissulaire (10). A l'étape suivante, le tissu (36) est découpé par un faisceau laser focalisé. La pellicule (14) est également sectionnée à cette occasion. La bande adhésive (20) est ensuite enlevée du microscope. Les parties découpées du tissu (36) adhèrent à la bande adhésive (20). Le reste de la pellicule (14) et de la coupe tissulaire (10) demeurent par adhérence sur la lame porte-objects du microscope (18).

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