System for regulating in vivo the expression of a transgene...

A - Human Necessities – 61 – K

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A61K 48/00 (2006.01) A01K 67/027 (2006.01) C12N 15/11 (2006.01) C12N 15/63 (2006.01) C12N 15/79 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2419790

The invention concerns novel constructs, compositions and a novel method for regulating in vivo the expression of a transgene of interest by conditional inhibition, and their uses for experimental, clinical and therapeutic purposes or for producing animals or plants. More particularly, the novel regulating method consists in the co-expression of a transgene of interest coding for a transcript of interest and an inhibitor transgene coding for an inhibitor transcript specific of the transcript of interest, so as to obtain constitutive inhibition of the activity of the transcript of interest, and so as to be able to ensure an efficient regulation of the transcript of interest, either by inhibiting its inhibitor transcript, or by activating the transcript of interest, or still by activating the transcript of interest and simultaneous inhibiting of its inhibitor transcript.

La présente invention concerne de nouvelles constructions, compositions et un nouveau procédé pour la régulation de l'expression d'un transgène d'intérêt in vivo par inhibition conditionnelle, ainsi que leurs utilisations dans les domaines expérimentaux, cliniques, thérapeutiques, ou pour la production d'animaux ou végétaux. Plus particulièrement, le nouveau procédé de régulation repose sur la coexpression d'un transgène d'intérêt codant pour un transcrit d'intérêt et d'un transgène inhibiteur codant pour un transcrit inhibiteur spécifique du transcrit d'intérêt, de sorte à obtenir une inhibition constitutive de l'activité du transcrit d'intérêt, et à pouvoir assurer une régulation efficace du transcrit d'intérêt, soit par inhibition de son transcrit inhibiteur, soit par activation du transcrit d'intérêt, soit encore par activation du transcrit d'intérêt et inhibition concomitante de son transcrit inhibiteur.

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