Use of baculovirus early promoters for expression of foreign...

Details Use of baculovirus early promoters for expression of foreign... Use of baculovirus early promoters for expression of foreign...

C - Chemistry, Metallurgy

12

N

C12N 15/86 (2006.01) C12N 5/10 (2006.01) C12N 9/72 (2006.01) C12N 15/866 (2006.01) C12N 15/87 (2006.01)

Patent

CA 2091887

2091887 9205265 PCTABS00011 The present invention provides an alternative strategy for baculovirus-mediated foreign gene expression: the promoters from immediate-early or delayed-early baculovirus viral genes were used to obtain the continuous expression of foreign genes in stably-transformed insect cells. The IE1 or the 39K promoters from Autographa californica nuclear polyhedrosis virus can drive the continuous expression of a foreign gene in insect cells with the advantages of more efficient processing, and minimal degradation of recombinant protein products. The recombinant baculoviruses are highly infectious in vivo and resistant to inactivation in nature. Therefore, this virus would be very effective as a delivery system of pesticides.

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