Increasing the sensitivity in the immunochemical...

G - Physics – 01 – N

Patent

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G01N 33/543 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2210916

The present invention relates to a process for the determination of an analyte, in which a sample optionally containing the analyte to be determined is brought into contact with a first receptor R1, which has a binding affinity for the analyte, immobilized on a solid phase, and a receptor R2, which likewise has a binding affinity for the analyte, is added and in which furthermore the resulting immune complexes are brought into contact with a binding factor which has a binding affinity for the analyte and for a receptor R3 immobilized on the same or a second solid phase and in which the amount of the analyte bound to the first solid phase or, if present, to the second solid phase is detected in a suitable manner.

La présente invention concerne un procédé pour le dosage d'un analyte, dans lequel un échantillon contenant facultativement l'analyte à doser est mis en contact avec un premier récepteur R1, qui a une affinité de liaison pour l'analyte, immobilisé dans une phase solide; un récepteur R2, qui a également une affinité de liaison pour l'analyte, est ajouté; les complexes immuns ainsi obtenus sont mis en contact avec un facteur de liaison qui possède une affinité de liaison pour l'analyte et pour un récepteur R3 immobilisé dans la même phase solide ou dans une seconde phase solide; la quantité d'analyte lié à la première phase solide ou à la seconde phase solide, selon le cas, est détectée de manière appropriée.

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