Human-derived tumor cell growth inhibitors

Details Human-derived tumor cell growth inhibitors Human-derived tumor cell growth inhibitors

C - Chemistry, Metallurgy

12

N

C12N 15/19 (2006.01) C07K 14/47 (2006.01) C07K 14/52 (2006.01) C12N 1/21 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2164295

A DNA fragment encoding a novel human-derived tumor cell growth inhibitor can be obtained from a cDNA library originating from human colonel tumor cell, using as a DNA probe a DNA fragment encoding mouse human-derived tumor cell growth inhibitor. An expression plasmid bearing a promoter originating from Bacillus brevis, a signal peptide originating from Bacillus brevis and a DNA fragment encoding human- derived tumor cell growth inhibitor downstream of the signal peptide is constructed and Bacillus brevis is transformed by the expression plasmid. The resulting transformant is incubated to secrete the novel inhibitor out of the cell so that the novel inhibitor can be produced efficiently.

Il est possible d'obtenir un fragment d'ADN codant un nouveau facteur inhibiteur de prolifération de cellules tumorales d'orgine humaine à partir d'une librairie d'ADNc provenant de cellules de cancer du colon chez l'homme en utilisant un fragment d'ADN codant un facteur inhibiteur de prolifération de cellules tumorales chez la souris comme sonde d'ADN. Il est possible d'obtenir à partir des cellules la sécrétion d'un nouveau facteur inhibiteur de prolifération de cellules tumorales d'origine humaine, en construisant un plasmide d'expression renfermant un promoteur provenant de Bacillus brevis, un peptide signal provenant de Bacillus brevis et, en aval, un fragment d'ADN codant ledit nouveau facteur inhibiteur, en transformant Bacillus brevis par utilisation du plasmide susvisé et en cultivant le transformant ainsi obtenu. On obtient ainsi de manière efficace ce nouveau facteur inhibiteur de prolifération.

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